Generator

Part:BBa_K395706:Design

Designed by: Yumiko Kinoshita   Group: iGEM10_Tokyo_Tech   (2010-10-08)


rbs+crtZW under pBad promoter for double plasmids


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2405
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2488
    Illegal SapI site found at 961
    Illegal SapI site found at 2315
    Illegal SapI site found at 2327


Design Notes

・This operon is under arabinose inducible promoter.

・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project.



Source

CrtZ comes from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.

CrtW comes from Brevundimonas sp. strain SD212 (MBIC 03018) (Accession number AB377271).

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This Composite Biobrick is created by standard assembly of Part BBa_I0500, Part BBa_I742158 and Part BBa_K395705.


References